one of our recent projects we built an combined atomic force microscope
and total internal reflection microscope. In collaboration with Pedro
de Pablo (Universidad
Autónoma de Madrid) and Urs Greber (University of Zurich)
we used this instrument to study the the genome release of viruses (Ortega-Esteban et al
AFM unpacking of adenovirus
cartoon of the experiment shows the unpacking experiment. By using
Yoyo-1 as fluorescent label we can track the release of the viral DNA.
The full animation can be found here.