one of our recent projects we combined atomic force microscopy with total internal reflection microscope to
study the the genome release of viruses (Ortega-Esteban et al
. 2015). This work was performed in collaboration with Pedro
de Pablo (Universidad
Autónoma de Madrid) and Urs Greber (University of Zurich)
AFM unpacking of adenovirus
cartoon of the experiment shows the unpacking experiment. By using
Yoyo-1 as fluorescent label we can track the release of the viral DNA.
The full animation can be found here.